TABLE 2.

Changes in MICs in relationship to Mcc24 exposure in a representative experiment

Treatment	Straina	MIC (μg/ml) ofb:				Zone of inhibition around MC4100pGOB18 disk
		CIP	TET	CHL	RIF
Two exposures to disks soaked in MC4100pGOB18	SL1344/CFP/marR border colonies	0.125	4	2	12.5	Yes
17 exposures to disks soaked in MC4100pGOB18	SL1344/CFP/marR border colonies	0.25	ND	ND	ND	Yes
18 exposures to disks soaked in MC4100pGOB18	SL1344/CFP/marR border colonies	2	16	32	50	No
17 exposures to disks soaked in MC4100pGOB18 followed by another exposure in the presence of 0.2% arabinose	SL1344/CFP/marR border colonies	0.5	ND	ND	ND	Yes
17 exposures to disks soaked in MC4100pGOB18 and then two more exposures in the presence of 0.2% arabinose	SL1344/CFP/marR border colonies	0.25	ND	ND	ND	Yes
17 exposures to disks soaked in MC4100pGOB18 one exposure to 0.2% arabinose, and then 2 to 3 additional exposures to disks soaked in MC4100pGOB18	SL1344/CFP/marR border colonies	2	16	32	50	No
18 expansions without exposure to disks soaked in MC4100pGOB18	SL1344/CFP/marR edge colonies	0.125	4	2	12.5	Yes
50 expansions without exposure to disks soaked in MC4100pGOB18	SL1344/CFP/marR edge colonies	0.125	4	2	12.5	Yes

^a“Border” indicates that colonies were selected from the border of the zone of inhibition, and “edge” indicates that colonies were selected from the edge of the agar plate. 

^bConcentrations of antibiotics were as described in Table 1, note a. ND, not determined.