TABLE 1.
S. meliloti strains: supplements required for growth in minimal medium
| Strain | Source | Growth in M9 medium (A620)a with:
|
||
|---|---|---|---|---|
| None | Yeast extract | Met + Cys + YE biotin | ||
| JJ1c10 | V. N. Iyer, Carleton University, Ottawa, Canada | 0.03 | 1.45 | 1.42 |
| 1021 | T. M. Finan, McMaster University, Hamilton, Canada | 0.03 | 1.11 | 1.45 |
| NRG185 | W. A. Rice, Beaverlodge Research Station | 0.02 | 0.93 | 0.96 |
| 102F34 | J. C. Burton, Nitragin Co., Milwaukee, Wis. | 0.02 | 0.97 | 0.75 |
| Balsac | L. M. Bordeleau, Sainte-Foy Research Station | 0.00 | 0.71 | 0.32 |
| Rm41 | A. Kondorosi, Szeged, Hungary | 0.03 | 1.00 | 0.85 |
| Elora2 | J. C. Sirois, CBRI, Ottawa, Canada | 0.01 | 0.58 | 0.98 |
| Kirby2 | J. C. Sirois, CBRI, Ottawa, Canada | 0.00 | 0.58 | 0.67 |
| R304 | M. R. Purdom, Marandellas, Zimbabwe | 0.01 | 0.39 | 1.17 |
| R423 | M. R. Purdom, Marandellas, Zimbabwe | 0.00 | 0.42 | 0.93 |
| 2012 | M. Dye, Rothamsted Experimental Station | 0.00 | 0.44 | 0.68 |
| DMG117 | C. T. Corke, University of Guelph, Guelph, Canada | 0.02 | 0.60 | 1.29 |
| DMG118 | C. T. Corke, University of Guelph, Guelph, Canada | 0.01 | 0.64 | 1.27 |
| MB5 | S. M. Lesley, CBRI, Ottawa, Canada | 0.02 | 0.67 | 0.33 |
| Cavan2 | J. C. Sirois, CBRI, Ottawa, Canada | 0.02 | 0.64 | 0.49 |
| Harrow2 | J. C. Sirois, CBRI, Ottawa, Canada | 0.03 | 0.78 | 0.56 |
| Kirby5 | J. C. Sirois, CBRI, Ottawa, Canada | 0.07 | 0.83 | 0.81 |
| R762 | Our field isolate | 0.06 | 0.90 | 0.94 |
| U45 | J. Brockwell, CSIRO, Canberra, Australia | 0.03 | 0.55 | 0.97 |
| MB1 | S. M. Lesley, CBRI, Ottawa, Canada | 0.01 | 0.71 | 0.79 |
| MB6 | S. M. Lesley, CBRI, Ottawa, Canada | 0.02 | 0.56 | 0.67 |
| MBA25 | S. M. Lesley, CBRI, Ottawa, Canada | 0.02 | 0.54 | 0.93 |
| BT5 | S. M. Lesley, CBRI, Ottawa, Canada | 0.06 | 0.21 | 0.79 |
| YG2 | S. M. Lesley, CBRI, Ottawa, Canada | 0.02 | 0.72 | 0.86 |
| 1642 | E. Bromfield, PRC, Ottawa, Canada | 0.02 | 0.73 | 1.17 |
| R763 | Our field isolate | 0.02 | 0.86 | 0.74 |
| I-6-5 | S. M. Lesley, CBRI, Ottawa, Canada | 0.01 | 0.57 | 0.57 |
a
Each strain was tested by inoculating a minimal medium culture with cells from minimal M9 agar. The culture was subdivided, additionally supplemented as indicated, and shaken at 30°C for 2 to 3 days before growth was monitored. Values shown are the averages of three measurements (standard deviation < 0.01). None, no additional supplements; Yeast extract, 50 μg of yeast extract per ml; Met + Cys + YE biotin, 5 μg of each of methionine and cysteine per ml and 5 ng of biotin purified from yeast extract per ml.