Table 2.

The detection conditions of HPLC for each CYP450 isoenzymes

cytochromes	Internal reference	Mobile phase	wavelength
CYP1A2	7-Hydroxycoumarin	Methanol: phosphate buffer (pH = 3.0, 50 mM) = 32:68	UV 245 nm
CYP2A6	-	Acetonitrile: acetic acid (0.1%, v/v) = 35:65	Fluo Ex/EM 340/456 nm
CYP2B6	Hydroxybupropion	Acetonitrile: water (v/v) = 20:80 for 2.5 min, 90–10 for 4 min	UV 268 nm
CYP2C8	-	Methanol: Water = 65:35	UV 230 nm
CYP2C9	Coumarin	Acetonitrile (A): phosphate buffer (pH = 7.4, 100 mM, B) = 32:68, 0–9 min, 68% B-32% B	UV 280 nm
CYP2C19	Tolbutamide	Methanol: potassium phosphate (pH 7.0, 10 mM) = 30:70	UV 204 nm
CYP2D6	-	Acetonitrile: phosphate buffer (pH = 3.0, 50 mM) = 25:75	Fluo Ex/EM 235/310 nm
CYP2E1	Phenacetin	Acetonitrile: acetic acid (0.5%, v/v) = 22:78, 1–10 min, 78% B-40% B	UV 287 nm
CYP3A4	Corticosterone	Methanol: water = 50:40, 0–15 min, 48% B-30% B; 15–22 min, 30% B-20% B	UV 254 nm