KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| α-Synuclein | Abcam | ab138501 (MJFR1) |
| α-Synuclein | BD Bioscience | 610787; AB_398107 |
| Phospho Ser129 α-synuclein | Abcam | ab51253 (EP1536Y) |
| Tyrosine hydroxylase (TH) | Pel-Freeze Biologicals | P40101 |
| PPARα | Santacruz | sc-398394 |
| PPARβ | Santacruz | sc-74517 |
| PPARγ | Santacruz | sc-7273 |
| PGC1α | Millipore | AB3242 |
| TFEB | Abcam | ab2636 |
| Lamp2 | Millipore | MABC40; AB_2662613 |
| Cathepsin D | Santacruz | sc-6487 |
| TPP1 | Abcam | ab54685 |
| Actin | Abcam | ab8226 |
| Chemicals, peptides, and recombinant proteins | ||
| Recombinant human α-syn | Anaspec | AS-1000 |
| Fenofibrate | Millipore Sigma | F6020-100G |
| SYBR green real-time kit | QuantaBio | K1070 |
| Proteinase K | Sigma | 3115828001 |
| IRDye infrared dye end-labeled oligonucleotide probe for PPAR | Li-COR Biosciences | Custom made |
| Deposited data | ||
| Raw and analyzed data | Figshare | https://doi.org/10.6084/m9.figshare.20032727 |
| Experimental models: Organisms/strains | ||
| M83 (A53T) | Jackson Laboratory | B6;C3-Tg(Prnp-SNCA*A53T)83Vle/J |
| CLN2−/− | Ghosh et al., 2017 | https://doi.org/10.1111/jnc.13987 |
| C57BL6 | Envigo | C57BL/6JOlaHsd |
| PPARα−/− | Jackson Laboratory | B6; 129S4-Pparatm1Gonz/J |
| Oligonucleotides | ||
| Primers for lysosomal genes, see Table S2 | This paper | NA |
| Primer corresponding to Tfeb promoter, sense: 5′-GAA CAT TCC AGG TGG AGG CA-3′ | This paper | NA |
| Primer corresponding to Tfeb promoter, antisense: 5′-CCC CCA ACA CAT GCT TCT CT-3′ | This paper | NA |
| Software and algorithms | ||
| Fiji (ImageJ2) | Schneider et al., 2012 | https://doi.org/10.1038/nmeth.2089 |
| Morpheus | Broad Institute | https://software.broadinstitute.org/morpheus/ |
| GraphPad Prism v7.02 | GraphPad Software Inc. | https://www.graphpad.com/scientific-software/prism/ |