KEY RESOURCES TABLE
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
α-Synuclein | Abcam | ab138501 (MJFR1) |
α-Synuclein | BD Bioscience | 610787; AB_398107 |
Phospho Ser129 α-synuclein | Abcam | ab51253 (EP1536Y) |
Tyrosine hydroxylase (TH) | Pel-Freeze Biologicals | P40101 |
PPARα | Santacruz | sc-398394 |
PPARβ | Santacruz | sc-74517 |
PPARγ | Santacruz | sc-7273 |
PGC1α | Millipore | AB3242 |
TFEB | Abcam | ab2636 |
Lamp2 | Millipore | MABC40; AB_2662613 |
Cathepsin D | Santacruz | sc-6487 |
TPP1 | Abcam | ab54685 |
Actin | Abcam | ab8226 |
Chemicals, peptides, and recombinant proteins | ||
Recombinant human α-syn | Anaspec | AS-1000 |
Fenofibrate | Millipore Sigma | F6020-100G |
SYBR green real-time kit | QuantaBio | K1070 |
Proteinase K | Sigma | 3115828001 |
IRDye infrared dye end-labeled oligonucleotide probe for PPAR | Li-COR Biosciences | Custom made |
Deposited data | ||
Raw and analyzed data | Figshare | https://doi.org/10.6084/m9.figshare.20032727 |
Experimental models: Organisms/strains | ||
M83 (A53T) | Jackson Laboratory | B6;C3-Tg(Prnp-SNCA*A53T)83Vle/J |
CLN2−/− | Ghosh et al., 2017 | https://doi.org/10.1111/jnc.13987 |
C57BL6 | Envigo | C57BL/6JOlaHsd |
PPARα−/− | Jackson Laboratory | B6; 129S4-Pparatm1Gonz/J |
Oligonucleotides | ||
Primers for lysosomal genes, see Table S2 | This paper | NA |
Primer corresponding to Tfeb promoter, sense: 5′-GAA CAT TCC AGG TGG AGG CA-3′ | This paper | NA |
Primer corresponding to Tfeb promoter, antisense: 5′-CCC CCA ACA CAT GCT TCT CT-3′ | This paper | NA |
Software and algorithms | ||
Fiji (ImageJ2) | Schneider et al., 2012 | https://doi.org/10.1038/nmeth.2089 |
Morpheus | Broad Institute | https://software.broadinstitute.org/morpheus/ |
GraphPad Prism v7.02 | GraphPad Software Inc. | https://www.graphpad.com/scientific-software/prism/ |