Skip to main content
. 2022 Jun 22;7(12):e157215. doi: 10.1172/jci.insight.157215

Figure 6. Mouse rCXCL13 induces TLS in tumors and prolongs survival.

Figure 6

(A) Mouse rCXCL13 was administered i.p. to induce TLS in a mouse ovarian cancer model. Representative H&E images of TLS formed in an omental tumor. The area of TLS per tumor area was compared between the control group and the rCXCL13 treated group (n = 7, each). Statistical significance was determined by 2-tailed Student’s t test. *P < 0.05. (B) TLS induced by mouse rCXCL13 (H&E) and expression of mouse CXCL13 corresponding to TLS (RNA ISH, Fast RED). (C) CD8+ T cell IHC images (DAB) in the rCXCL13-treated group and control group. Scale bars: 100 μm. (D and E) The effect of rCXCL13 administration on the survival of tumor-bearing mice was compared between immunocompetent mice (D) and immunodeficient mice (E). Analyses were performed using Kaplan-Meier estimates and log-rank tests.