Skip to main content
. 2022 Jun 22;7(12):e155338. doi: 10.1172/jci.insight.155338

Figure 2. Deletion of Cth reduces gastritis in an acute model of H. pylori pathogenesis.

Figure 2

WT and Cth–/– mice were infected or not with H. pylori PMSS1 for 8 weeks. (A) CTH mRNA expression in gastric tissues of WT and Cth–/– mice infected or not with H. pylori; n = 3–7 mice per genotype. (B) Representative immunofluorescence images of CTH (green) colocalized (yellow) with the macrophage marker CD68 (red) from WT and Cth–/– mice infected or not with H. pylori; n = 3 mice per genotype. DAPI (blue). (C) H&E images from infected mice; arrowheads highlight inflammatory cells. (D) Histologic gastritis scores. Each symbol is a different mouse; data are pooled from 2 independent experiments. (E) H. pylori colonization in gastric tissues from D. (F) Correlation between gastritis in D and H. pylori colonization in E. All values are reported as mean ± SEM. Statistical analyses, where shown, are: (A and D) 1-way ANOVA with Kruskal-Wallis test, followed by a Mann-Whitney U test; (E) Student’s t test; (F) correlation and significance determined by Pearson’s product-moment correlation test. *P < 0.05, **P < 0.01, ****P < 0.0001. (B and C) Scale bars: 50 μm. Ctrl, control.