Figure 3.
Loss of Metrnl expression does not affect skeletal development. Metrnl+/− × Metrnl+/− breeding pairs were used to generate wildtype, haplotype, and knockout progeny. Skeletons and femurs of (A) E15.5 mice and (B) E18.5 mice underwent whole mount staining (Alizarin red, bone; Alcian blue, cartilage). Relative femur length and relative mineralization length of E15.5 pups (C,D) and E18.5 pups (E,F) was measured. n = 5, data are expressed as mean ± 95% confidence interval [Color figure can be viewed at wileyonlinelibrary.com]