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. 2022 Feb 8;40(11):2510–2521. doi: 10.1002/jor.25286

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© 2022 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited and no modifications or adaptations are made.

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Metrnl expression is dispensable for normal bone fracture healing. (A) Metrnl expression was assessed within the fracture callus during repair. (B) IHC for Metrnl in 14‐day fracture calluses was performed. (C) 4‐month‐old Metrnl ^+/+, Metrnl ^+/−, and Metrnl^−/− mice underwent tibial fracture and fractured limbs were isolated 21 and 28 days postinjury. (D) 21‐day fracture calluses were investigated using micro‐CT analysis to determine (E) tissue mineral density (TMD) and (F) bone volume relative to total volume (BV/TV). (G) Histological sections of healing fractures were stained with Alcian Blue/Orange G/Hematoxylin. Mechanical testing was performed on limbs harvested 28 days after injury to determine (H) structural stiffness and (I) force to refracture. For IHC and rt‐pcr, n = 5. For μCT and histology, n = 10. Data are expressed as mean ± 95% confidence interval. μCT, micro‐computed tomography; IHC, immunohistochemistry [Color figure can be viewed at wileyonlinelibrary.com]