Figure 6.

Loss of Metrnl does not induce transcriptional changes associated with bone formation. (A) Integrated Genomics Viewer screenshot of representative RNA‐seq data from Metrnl ^+/+ and Metrnl^−/− bone specimens. Deletion of exon 2 results in an alternate transcription event, which introduces a premature stop codon, as shown in the diagram below. (B) Volcano plot depicting significant changes (red dots, p < 0.05 after correction for multiple hypothesis testing) in n = 35 transcripts due to loss of Metrnl expression. (C) Four representative transcripts with a significant change in abundance in Metrnl^−/− mouse diaphyseal bones [Color figure can be viewed at wileyonlinelibrary.com]