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. 2022 Feb 5;69(5):e12890. doi: 10.1111/jeu.12890

FIGURE 9.

FIGURE 9

Role of protein phosphorylation in specification of the division plane position in Tetrahymena. (A) Control cell, BBs labeled with anticentrin (Kaczanowska et al., 2012). Arrows indicate FZ. (B) A 6‐DMAP‐treated cells showing anterior displacement of FZ and OP (note unequal size of daughter cells; Kaczanowska et al., 1999). (C) A roscovitine‐treated cells showing posterior displacement of FZ (but not the OP; Kaczanowska et al., 2012). (D) A double mutant (cdaA1/ cdaI1) grown at restrictive temperature (Jiang et al., 2020). OP is displaced anterior to the FZ. (E) MPM‐2 immunostaining of triton‐extracted Tpyriformis, a gradient of phosphorylated protein epitopes posterior to the FZ [from Kaczanowska et al. (1999)].