Figure 1.

Efficient MC repopulation in neonatal NSG recipient mice. (A) Representative flow plots of recipient’s PerC cells transplanted with BM HSC cells. Sorted 100 adult BM HSCs or 3000 MPPs (B6; CD45.2) were transplanted into irradiated F1 B6/BoyJ (CD45.1+CD45.2+) mice together with 3x10⁵ BoyJ BM MNCs (CD45.1). (B) Percentages of the donor, competitor, and recipient MC chimerism in the recipient’s PerC. (n=5 each) (C) A gating strategy for sorting of c-Kit⁺β7-integrin⁺ MC progenitor cells from E12.5 B6 FL (Top Panel). Representative gating for MC and macrophage populations in PerC of recipient NSG mice 2 months after transplant with 3500 of Kit⁺β7-integrin⁺ cells (bottom panel). (D) % reconstitution of MCs and Macrophages population in PerC of recipient NSG mice as in C(n=4).