Figure 5.

Diversification of HSC to the MC progenitor and HSC maturation. E10.5–11.5 pre-HSC in the AGM region contains a variety of cell populations including HSC with MC capacity (plus other innate lineages too), MPP, and other lineage-restricted progenitors. E12.5 FL contains at least 3 classes of MC repopulating cells; 1) HSCs (with reduced MC differentiation capacity), 2) MPPs with MC potential, and 3) Kit⁺β7-integrin⁺ MC progenitor cells. 1) is the consequence of E11.5 pre-HSC/HSC and 2) are derived from E9.5-10.5 AGM EC-derived production. 3) are derived from the early EC-derived wave and later AGM EC wave. In E14.5, HSCs had lost MC capacity, but MC progenitors still exist within MNCs, then distribute into general tissue and maintain long term in local.