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. 2022 Jul 8;12:894043. doi: 10.3389/fonc.2022.894043

Figure 4.

Figure 4

FOXS1 is involved in tumor cell-induced angiogenesis. (A) HUVEC tube formation after stimulation with the indicated conditional medium from vector control CRC cells and FOXS1-overexpressed CRC cells. Scale bars: 50 µm. The number of tube junctions was calculated (right). (B) Migration of HUVECs toward conditioned medium from vector control CRC cells and FOXS1-overexpressed CRC cells. Scale bars: 50 µm. The number of migrated HUVECs was calculated. (C) Representative images of the CAM assay. Histograms show the formation of secondary and tertiary blood vessels after stimulation with the indicated conditional medium from FOXS1-overexpressing cells. (D) HUVEC tube formation after stimulation with the indicated conditional medium from FOXS1-depleted CRC cells. Scale bar: 50 µm. The number of tube junctions was calculated (right). (E) Migration of HUVECs toward conditioned medium from FOXS1-depleted CRC cells. The number of migrated HUVECs was calculated. Scale bar: 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001.