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Images of plates for a successful conjugation. (A) Lawn of spores as should be expected in Step B.1. (B) A typical plate after an overnight incubation prior to antibiotic overlay as in Step C.9. (C) A plate containing S. coelicolor conjugates after 5 days after adding antibiotics as in Step C.11. (D) A succesful streak of 4 spore colonies onto MS-agar containing apramycin and nalidixic acid as in Step C.14.
