FIGURE 2.

Fitness of evolving populations at 6 time points from lineage 1, 2, and 3. The growth curve (a), the area under the curve (b), and the maximum OD₆₀₀ (c) of each population were compared with the same data of ancestral strain ATCC 17978/pAZJ221 in imipenem‐containing MH broth. Data were collected for 20 h at 37 °C with shaking. Statistical analysis was performed through Dunnett's multiple comparisons test with Dunnett's T3 multiple comparisons test for unequal variances. *, adjusted p value < 0.05; ns, not statistically significant