FIGURE 9.

Conjugation frequencies (a) and log₁₀conjugation rates (b) of the ancestor strain ATCC 17978/pAZJ221, six mutants with pAZJ221, and ATCC 17978/pAZJ221E. The data of each mutant were compared to that of the ancestor strain ATCC 17978/pAZJ221. Statistical analysis was performed through Dunnett's multiple comparisons test. **, adjusted p value < 0.01; ****, adjusted p value < 0.0001; ns, not statistically significant. (c) The plasmid‐containing cells percentage of ATCC 17978/pAZJ221 and six mutants with pAZJ221 were assessed on the first day and the tenth day of propagation. Statistical analyses were performed through unpaired t‐tests. *p < 0.05; Wild Type, ATCC 17978