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. 2022 Jul 11;13:833540. doi: 10.3389/fphys.2022.833540

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Copyright © 2022 Lang, Nguyen, Bhadra, Jung, Helms and Zimmermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Physicochemical properties of Sec62/Sec63 clients. (A–C) We used custom scripts to compute the hydrophobicity of SPs (A,B) and SP H-regions (C,D), respectively. Hydrophobicity score was calculated as the averaged hydrophobicity of its amino acids according to the Kyte-Doolittle propensity scale. For the calculation of H-region hydrophobicity, each SP was subjected to segmentation using the prediction tool Phobius (https://phobius.sbc.su.se) and the H-region to the calculation according to Kyte-Doolittle. (E) Relevant properties of SPs (hydrophobicity and positively charged amino acid residues, respectively) and mature regions of four clients. Hydrophobicity scores were calculated according to the Kyte-Doolittle propensity scale and are displayed using the DNAstar software package. Apparent delta G values were determined with the ΔG_app predictor for TM helix insertion (http://dgpred.cbr.su.se). Clusters of positive charges in the respective mature region that were experimentally shown to contribute to Sec62/Sec63 dependence are indicated.