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. 2022 Jul 11;13:833540. doi: 10.3389/fphys.2022.833540

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Copyright © 2022 Lang, Nguyen, Bhadra, Jung, Helms and Zimmermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Physicochemical properties of SPs of TRAP clients. (A–C) We used custom scripts to compute the hydrophobicity score (A), apparent delta G (B), and glycine/proline (GP) content (C) of SP sequences. Hydrophobicity score was calculated as the averaged hydrophobicity of its amino acids according to the well-known Kyte-Doolittle propensity scale. Apparent delta G values were determined with the ΔG_app predictor for TM helix insertion (http://dgpred.cbr.su.se). GP content was calculated as the total fraction of glycine and proline in the respective sequence. (D) We also used custom scripts to extract protein annotations for all human, E. coli and S. cerevisiae SPs from UniProtKB entries and to calculate their GP content. (E,F) Cartoon of unclipped (E) and clipped (F) 80S ribosome together with Sec61-complex and TRAP, and OST. Notably, without clipping eL38 and helix 51 is partially hidden.