FIGURE 6.

Venn diagram for components for precursor targeting to the human ER membrane and their clients. The diagram summarizes precursors with SP (in red) or TMH (in black), which are destined to the secretory pathway and were negatively affected by the indicated depletion in HeLa cells (Tirincsi et al., 2022b) or by PEX3 depletion in HeLa cells and after PEX3 knock-out in Zellweger patient fibroblasts (Zimmermann et al., 2021); membrane protein precursors with SP are underlined, as are TMH proteins with TA. All these precursor polypeptides are defined as potential clients or substrates of the respective component or complex. The percentage of membrane proteins (MP) of the secretory pathway among the respective clients is indicated (%). HLA-C is in italics to highlight that it was negatively affected by PEX3 as well as SRA and WRB depletion, likewise are highlighted FAR1 and HLA-A, HLA-B, ITPR3 plus TMEM41B, respectively. Asterisks indicate hairpin proteins.