FIGURE 9.

Venn diagram for human ER protein translocation components and their clients. The diagram summarizes precursors with SP (in red) or TMH (in black), which are destined to the secretory pathway and were negatively affected by the indicated depletion; membrane protein precursors with SP are underlined, as are TMH proteins with TA. All these precursor polypeptides are defined as potential clients or substrates of the respective component or complex. The section for Sec62 and Sec63 summarizes the negatively affected proteins after the respective depletions in HeLa cells and the knock-outs in HEK293 cells, respectively (Schorr et al., 2020); the section for TRAM1 summarizes the negatively affected proteins after the TRAM1 depletion in HeLa cells (Klein et al., 2020); the section for TRAP summarizes the negatively affected proteins after the TRAPB depletion in HeLa cells and the TRAPD or TRAPG knock-outs in CDG patient fibroblasts (Nguyen et al., 2018). The percentage of membrane proteins (MP) of the secretory pathway among the respective clients is indicated (%). ADAM10, CNPY4, CYR61, ITRIP, LNPEP and TMEM223 are in italics to highlight that they were negatively affected by TRAM1 as well as TRAPB depletion.