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Measurements of Zn(II) binding affinity to HSA in the absence of FA. ITC data for Zn(II) binding to HSA in the absence of FA. The data was fitted to two set of binding sites. One high affinity site (MBS-A); KD1 = 5.43 ± 0.5 µM and up to seven secondary low affinity sites KD2 = 80 ± 5 µM was obtained.
