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Detection of BLV proviral DNA and anti-BLV antibodies. (Main) BLV FRET-qPCR showed that five and six inoculated cows were detected to be positive at 0 and 5 DPI, respectively. From 45 DPI, all cows in the intervention group were BLV-positive by FRET-qPCR and ELISA. In comparison, all the control cows were free of BLV throughout this study. (Insertion) The copy numbers of BLV pol gene of cows in the intervention and control groups were quantified by FRET-qPCR.
