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. 2022 Jul 11;16:924603. doi: 10.3389/fnbeh.2022.924603

FIGURE 2.

FIGURE 2

(A–D) Wellbeing parameters. (A) Burrowing test. The burrowed gravel in percentage over the training week is expressed as the mean. The DAT KO rats burrowed significantly lower amounts of gravel on all days. Sample Size: DAT KO (n = 16), DAT HET (n = 20), WT (n = 20). (B) Saccharin preference test. The bars show the mean preference of the saccharin solution over water over both testing days. The preference for the saccharin solution is significantly lower for the DAT KO rats. Sample Size: DAT KO (n = 8), DAT HET (n = 10) and WT rats (n = 10). (C,D) Social interaction. (C) Frequency of passive and active interactions shown during the first 5 min of the social interaction test. The DAT KO rats displayed less active interactions compared to the DAT HET and WT rats. No differences were observed in the frequency of passive interactions. (D) Duration of the shown active and passive interactions shown during the first 5 min of the social interaction test. The DAT KO rats spent significantly more time interacting passively and less time with active interactions compared to the two other genotypes. (C,D) Data are expressed as the mean + SEM. Sample size for all genotypes n = 8. (A–D) The dots in the bar graphs indicate all included data points in the analysis. *p < 0.05, **p < 0.01, and ***p < 0.001.