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PCR amplification of genomic DNA from E. huxleyi strain 1516 with primers specific for the E. huxleyi RubisCO large-subunit gene. DNA extracted from single-colony isolates on F/50 plates (lanes 2 and 3) and from liquid-grown cells (lanes 4 and 5) produced the same 1.2-kb fragment. Subsequent sequencing of these PCR products confirmed the presence of the RubisCO large-subunit gene of E. huxleyi. Lane 1, 23-kb ladder.
