Skip to main content
. 2022 Jul 11;12:899987. doi: 10.3389/fonc.2022.899987

Figure 3.

Figure 3

USP13-dependent FASN expression promotes SCLC stemness and lipogenesis. (A) Immunoprecipitation and immunoblot analyses were performed with the indicated antibodies in H1048 cells. (B) Immunofluorescence analyses were performed with the indicated antibodies in H1048 cells. DAPI was used for nuclear staining. Scale bars: 20 μm. (C, D) FASN expression levels were detected in H1048 (top) or H69 (bottom) cells with or without USP13 depletion (C) or overexpression (D) by immunoblot analyses with an anti-FASN antibody. (E) H1048 cells with or without USP13 shRNA expression, or USP13 depletion reconstituted expression of WT HA-rUSP13 with or without FASN depletion were analyzed by immunoblotting with the indicated antibodies. (F) Left: ELDA was performed in H1048 cells with or without USP13 shRNA expression, or USP13 depletion combined with reconstituted expression of WT HA-rUSP13 with or without FASN depletion. Representative sphere images (top right) and stemness frequency illustration of the cells with the upper and lower 95% confidence intervals (bottom right) are shown. Scale bars, 50 μm. Data represent the means ± SD of wells. Two-tailed student’s t-test was used. ***p < 0.001. (G) Cellular triglyceride levels (left) and cholesterol levels (right) in H1048 cells with or without USP13 shRNA expression, or USP13 depletion combined expression of reconstituted expression of WT HA-rUSP13 with or without FASN depletion were determined. Data shown are the mean ± S.D. (n=3). Two-tailed student’s t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001.