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Averaged sum intensity for each parameter. Each bar is shown as sum intensity of fatty acid FA(18:2) m/z 279.23 and phosphatidylinositol PI(20:4/18:0) m/z 885.55, under each condition. The following parameters were tested: distance between emitter and orifice in μm; orifice diameter in μm; voltage in kV; solvent flow rate in μL/min; gas pressure in bar; shape of the distal tip and inner diameter of emitter in μm; solvent; and grounding of metal cap.
