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. 2022 Jul 5;94(28):10035–10044. doi: 10.1021/acs.analchem.2c00345

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© 2022 The Authors. Published by American Chemical Society

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(A) Overlay ion images of phosphatidylethanolamine PE(O-36:2) 728.56 m/z (red), fatty acid FA(22:6) 327.23 m/z (blue), and fatty acid FA(20:4) 303.23 m/z (green), with a pixel resolution of 50μm (top) and 25μm (bottom), compared between DESI (left) and DEFFI (right), on sagittal sections of a mouse brain, acquired in a negative ion mode. (B) Ion image for 3 low-intensity neurotransmitters in mouse brain: GABA 102.06 m/z (red), glutamine 145.06 m/z (green), and glutamate 146.05 m/z (blue), compared between DESI and DEFFI, on a mouse brain with a pixel resolution of 25μm.