Motility assay on semisolid medium plates. 1, Pseudomonas strain Y1000; 2, Enterobacter strain 22. Assays were performed at 25°C (A), at 25°C in the presence of 500 mM NaCl (B), at 37°C (C), or at 4°C (D). Plates were incubated for 13 to 15 h at 25 and 37°C and for 132 h at 4°C. The results are representative of those from three independent experiments.