Fig. 6.

DNP measurement of C. albicans samples. (A) Spectral deconvolution of 1D ¹³C CP spectra measured on C. albicans samples prepared in liquid cultures (left) and solid media (right). The spectra are measured on a 600 MHz/395 GHz DNP system. The simulated spectra (red) match well with the experimentally measured spectra (blue). Underneath are many individual lines that add up to the simulated spectra. (B) 2D ¹³C refocused INADEQUATE spectra of C. albicans. The left panel shows the CP refocused dipolar-INADEQUATE spectrum of unlabeled liquid culture measured using 600 MHz/395 GHz DNP (black grey) and the right panel shows the same type of spectrum collected on unlabeled C. albicans materials grown in solid media. For each panel, the DNP spectrum is overlaid with two high-resolution 2D spectra measured on ¹³C-labeled liquid culture at room temperature (RT), including a DP refocused J-INADEQUATE spectrum measured on an 800 MHz NMR (blue) and a CP refocused dipolar-INADEQUATE spectrum measured on a 400 MHz ss NMR (yellow). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)