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. 2022 Jul 11;13:925913. doi: 10.3389/fgene.2022.925913

FIGURE 1.

FIGURE 1

Overview of editor binding sites and relevant naturally occurring mutations relative to the target region within the PMEL and PRLR genes. (A) Shown is the DNA and amino acid sequence of the PMEL target region with the location of the ΔCTT (p.Leu18del) mutation. Binding sites of gRNA/Cas9 129F and TALEN pair TL17/TR217 are indicated by the blue and yellow boxes, respectively. The black bar indicates the protospacer adjacent motif (PAM) sequence of the gRNA/Cas9 editor. (B) Equivalent information for the location of three PRLR mutations T > A (p.C440*), ΔC (p.L462*), C > A (p.S465*) and binding and PAM sites of the gRNA/Cas9 editors 631, 632 and 634 in relation to the PRLR sequence.