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Effect of time of injection on HDR editing with plasmid-encoded gRNA 634/Cas9 in bovine embryos.
| Injection Time | Injection Sessions | No. Blastocysts | HDR + ve Embryos | 100% HDR |
|---|---|---|---|---|
| 5–6 h post IVF | 3 | 33 | 16/33 (49%) | 1/33 (3%) |
| 6–7 h post IVF | 3 | 36 | 22/36 (61%) | 1/36 (3%) |
| 7–8 h post IVF | 2 | 26 | 16/26 (62%) | 0/26 (0%) |
| 8–9 h post IVF | 3 | 17 | 10/17 (59%) | 0/17 (0%) |
