Table 1.
Target gene | Primer sequence (5ʹ→3ʹ) |
---|---|
GAPDH | F: AGGTCGGAGTCAACGGATTT R: TGACGGTGCCATGGAATTTG |
METTL3 | F: CTTGCATGGATTCTGAGGCC R: GTCAGCCATCACAACTGCAA |
METTL14 | F: AATCGCCTCCTCCCAAATCT R: CCACCTCTTTCTCCTCGGAA |
WTAP | F: TCAGTGCGGGGTATGAAAGT R: ACCTTTCCCACTCACTGCTT |
ALKBH5 | F: TCATCAACGACTACCAGCCC R: GGCTTGAACTGGAACTTGCA |
FTO | F: AGACACCTGGTTTGGCGATA R: CCAAGGTTCCTGTTGAGCAC |
YTHDF1 | F: GCAACTCTCCTGGAAACGTC R: TTGATGATGAACACACGCCC |
YTHDF2 | F: GTGTTGGAGAAGCTTCGGTC R: AGCAGCATCCAGTCTCTTGT |
YTHDF3 | F: CTGCCAAACCTCAACCGAAA R: GAGCCTTTACCACTGACCCT |
YTHDC1 | F: TTTCCTTCGTCGCACACAAG R: CGGTCTCTGTCTCGATCACA |
YTHDC2 | F: TCTGAGAATTGGGCTGTCGT R: TTCTCCTTTGGCCCTGTCAA |