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. 2022 Jul 25;13:4284. doi: 10.1038/s41467-022-31353-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a For the 3_6 pseudoknot, both the 77-nt and 144-nt PSM are shown in cartoon mode with their wildtype systems aligned in mesh (by PyMol⁷⁷ for the 77-nt region). The mutations are highlighted as spheres in pseudoknot-strengthening mutant (PSM) structure and labeled. The 2D structure comparison is also provided with PSM at top and wildtype at bottom. Comparisons for the (b) 77-nt 3_3 PSM and (c) 77-nt 3_5 mutant are shown in similar manner. Systems are numbered according to Table 1.