TABLE 3.
Effect of heat or trypsin treatment on cytotoxicity of cell-free supernatant fluids from selected Bacillus species for HEp-2 and Caco-2 epithelial cellsa
| Bacterial species | Isolate | % of the following cells dead after the indicated treatment:
|
|||||
|---|---|---|---|---|---|---|---|
| HEp-2
|
Caco-2
|
||||||
| Normal | HT | TT | Normal | HT | TT | ||
| B. cereus | KD4 | 76 ± 5 | 13 ± 1 | 11 ± 3 | 66 ± 3 | 10 ± 1 | 16 ± 3 |
| KD5 | 62 ± 11 | — | 5 ± 1 | 84 ± 9 | 80 ± 8 | 10 ± 2 | |
| KD10 | 83 ± 7 | 46 ± 3 | 22 ± 3 | 91 ± 7 | 60 ± 5 | — | |
| KD11 | 86 ± 5 | 35 ± 3 | 10 ± 1 | 83 ± 5 | — | 1 | |
| KD12 | 85 ± 2 | 6 | 8 ± 1 | 72 ± 6 | — | — | |
| Et | 85 ± 2 | 57 ± 5 | 3 ± 1 | 85 ± 7 | 2 | — | |
| Em | 83 ± 4 | 12 ± 3 | — | 75 ± 1 | — | — | |
| NR30 | 67 ± 5 | 15 ± 1 | — | 63 ± 2 | 15 ± 5 | — | |
| NR53 | 63 ± 1 | 22 ± 2 | — | 83 ± 4 | 4 | — | |
| NR11 | 65 ± 3 | — | — | 71 ± 10 | — | — | |
| B4ac | 65 ± 10 | 15 | — | 75 ± 7 | 28 ± 3 | 1 | |
| DC1 | — | — | — | — | — | — | |
| DC2 | 5 ± 1 | — | — | — | — | — | |
| B. licheniformis | KD1 | 67 ± 5 | — | — | 75 ± 2 | — | — |
| KD8 | 70 ± 6 | 8 ± 1 | — | 24 ± 2 | — | — | |
| KD17 | 68 ± 1 | 5 | 1 | 62 ± 10 | — | — | |
| H6 | 20 ± 2 | 3 | — | 36 ± 3 | — | — | |
| B. subtilis | KD19 | 49 ± 49 | 27 ± 3 | 1 | 72 ± 5 | 22 ± 2 | — |
| H7 | 85 ± 8 | 5 ± 1 | — | 62 ± 4 | — | — | |
| B. megaterium | KD16 | 56 ± 13 | 20 ± 5 | 3 | 35 ± 4 | — | — |
| NR73 | — | — | — | — | — | — | |
| B. sphaericus | KD18 | 61 ± 9 | 5 ± 2 | 1 | 52 ± 5 | 15 ± 3 | — |
| B. thuringiensis | KD3 | 66 ± 11 | 1 | — | 41 ± 5 | — | — |
| B. coagulans | KD13 | 15 ± 3 | 35 ± 6 | 9 ± 3 | — | — | 2 |
| B. circulans | KD9 | 57 ± 6 | 5 | — | 89 ± 2 | — | — |
| KD15 | 72 ± 2 | 35 ± 2 | 2 | 45 ± 5 | — | — | |
| H3 | 26 ± 2 | — | — | 5 ± 1 | — | — | |
| B. firmus | KD2 | 80 ± 11 | — | — | 42 ± 5 | — | — |
| KD7 | 63 ± 6 | — | — | 71 ± 5 | — | — | |
| B. brevis | H4 | 29 ± 10 | — | — | 15 ± 3 | — | — |
| B. lentus | H5 | 11 ± 2 | 3 | — | 17 ± 4 | — | — |
| B. mycoides | H1 | — | — | — | 5 | — | — |
| B. pumilus | KD14 | 48 ± 10 | 54 ± 3 | 12 ± 1 | 50 ± 4 | — | — |
| B. polymyxa | H2 | 59 ± 4 | 5 ± 1 | — | 79 ± 3 | — | — |
a
Supernatant fluids were untreated (Normal), heated (HT), or trypsin treated (TT) prior to assessment of toxicity to HEp-2 and CaCo-2 cells. Values shown are means and standard errors from triplicate trials where samples were examined in triplicate. —, no effect.