TABLE 4.

Screening of Bacillus strains for DNA sequences containing genes associated with the HBL complex and B. cereus BceT

Bacterial species	Isolate	Cytotoxicity (% cell death) in the following mediuma:					Detection of the following diarrheagenic gene by PCRb:
		BHI broth	IMF 1	IMF 2	IMF 3	IMF 4	bceT	hblC	hblA	hblD
B. cereus	KD4	76 ± 5	80 ± 5	83 ± 12	52 ± 5	50 ± 10	−	+	+	+
	KD5	62 ± 11	40 ± 3	72 ± 7	55 ± 3	47 ± 8	−	+	+	+
	KD10	83 ± 7	—	38 ± 3	53 ± 1	56 ± 3	+	+	+	+
	KD11	86 ± 5	66 ± 7	—	75 ± 6	88 ± 3	−	+	+	+
	NR11	85 ± 2	71 ± 9	38 ± 3	54 ± 5	17 ± 1	+	+	+	+
	NR14	63 ± 5	56 ± 7	74 ± 5	51 ± 3	62 ± 2	−	+	+	+
	NR42	45 ± 7	40 ± 5	55 ± 5	61 ± 4	56 ± 3	−	+	+	+
	NR50	86 ± 5	81 ± 4	75 ± 8	69 ± 4	61 ± 2	+	+	+	+
	NR93	81 ± 4	62 ± 5	73 ± 5	54 ± 8	66 ± 8	+	+	+	+
	B-4ac	75 ± 9	82 ± 3	64 ± 2	62 ± 5	55 ± 6	+	+	+	+
	Em	83 ± 4	45 ± 3	59 ± 5	62 ± 3	64 ± 4	−	−	−	−
B. licheniformis	KD1	67 ± 5	41 ± 5	29	—	—	+	+	+	+
	KD8	70 ± 6	64 ± 2	45 ± 3	55 ± 3	22 ± 3	+	+	+	+
	KD17	68 ± 1	57 ± 6	63 ± 2	60 ± 3	73 ± 8	−	−	−
	H6	20 ± 2	65 ± 3	52 ± 5	66 ± 9	68 ± 1	−	+	+	+
B. subtilis	KD19	49 ± 10	37 ± 3	30 ± 1	—	—	+	+	+	+
	H7	85 ± 8	77 ± 8	26 ± 4	70 ± 1	82 ± 3	−	−	−	−
B. pumilus	KD14	48 ± 10	53 ± 10	73 ± 5	43 ± 4	56 ± 1	−	−	−	−
B. circulans	KD9	57 ± 6	26 ± 3	—	29 ± 2	28 ± 1	−	+	+	+
	KD15	72 ± 2	5 ± 1	41 ± 3	45 ± 1	38	−	+	+	+
	H3	26 ± 2	47 ± 5	21 ± 4	8 ± 5	16 ± 3	+	−	−	−
B. thuringiensis	KD3	66 ± 11	55 ± 5	73 ± 8	—	—	+	−	−	−
B. megaterium	KD16	56 ± 13	44 ± 5	38 ± 3	42 ± 3	43 ± 2	+	+	+	+
	NR73	—	54 ± 6	43 ± 6	59 ± 3	65 ± 5	−	−	−	−
B. sphaericus	KD18	61 ± 13	38 ± 5	61 ± 5	64 ± 5	40 ± 5	−	−	+	+

^aValues are reported as means and standard errors. —, no effect. Samples that tested positive for diarrheal toxin production in the RPLA test kit are shown in bold. 

^b+, gene was detected; −, gene was not detected.