Fig. 2.

YKL-40 immunoreactivity and protein levels remain similar in post-mortem frontal cortex from FTLD and non-demented controls. A Representative images of paraffin sections from the frontal cortex stained with the anti-YKL-40 antibody. YKL-40 immunoreactivity is present in FTLD and control cases in glial and neuronal cells. Scale bars represent 50μM. Semi-quantitation of YKL-40 immunoreactivity was performed by grouping cases into either negative (i.e., zero or 1 positive cell) or positive (i.e., 2 or more positive cell groups). Stacked bar plots represent the percentage of cases with either negative (−, white area) or positive (+, black area) YKL-40 immunoreactivity in NDC (n = 7) and FTLD cases (n = 24; FTLD-Tau = 19 and FTLD-TDP = 5). B Representative immunoblot of YKL-40 in frontal cortex lysates. Actin was used as a loading control. Box-dot plots depict YKL-40 immunoblot reactivity corrected for actin in NDC (n = 14) and FTLD cases (n = 67; FTLD-Tau = 33, FTLD-TDP = 34). C YKL-40 levels were quantified by ELISA and corrected for total protein concentration in NDC (n = 14) and FTLD cases (n = 67; FTLD-Tau = 33 and FTLD-TDP = 34). Overall, no significant differences across groups were identified. Box represents median ± interquartile range with bars showing the lowest to highest points. Abbreviations: NDC, non-demented control; FTLD, frontal temporal lobar degeneration; n.s., non-significant difference between groups