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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Mol Cell Endocrinol. 2021 May 24;533:111332. doi: 10.1016/j.mce.2021.111332

Fig. 2: Effect of BPA, BPS, EE2 and their combinations on serum and testicular testosterone (T) concentrations in pubertal rats.

Fig. 2:

Male Long-Evans rats at 35 days of age were fed drinking water containing BPA, BPS, EE2 (5 μg/L) or their combinations (BPA+EE2, BPS+EE2, each at 2.5 μg/L) for 14 days. At sacrifice, blood was processed to obtain serum (A). Testicular explants were obtained and incubated in DMEM/Ham’s F-12 culture medium in triplicate without (basal, B) or with 100 ηg/ml ovine LH (NIDDK, NIH) (LH-stimulated, C) for 3 h. Aliquots of serum and spent media were analyzed to measure T concentrations by RIA (n = 6; *p < 0.05).