Testes were obtained from pubertal male Long-Evans rats (PND 34–49) fed drinking water containing BPA, BPS, EE2 (5 μg/L) or their combinations (BPA+EE2, BPS+EE2, each at 2.5 μg/L) for 14 days. Tissues were processed for western blot analysis to analyze 17β-hydroxysteroid dehydrogenase (Hsd17β) and cytochrome P45011A1 (Cyp11A1). Proteins were normalized to actin (ACTB). Tissues were obtained from three animals per group and western blot was repeated at least three times. Hsd17β=35 kDa, Cyp11A1=55 kDa, ACTB=43 kDa *, P<0.05 vs. control.