TABLE 1.

Characteristics of antibodies used

Target	Antibody sequence	Name of Antibody	Manufacturer, Catalog Number	Polyclonal or Monoclonal	Dilution Used
Amh (MIS)	Genetic locus: AMH (human) mapping to 19 p13.3; AMH (mouse) mapping to 10C1	MIS (C-20)	Santa Cruz Biotechnologies, Sc-6886 MW (AMH) 70/74 kDa	Mouse monoclonal IgG	1:2000
Dhh	Genetic locus: DHH (human) mapping to 12q13.12; Dhh (mouse) mapping to 15F1	Dhh (F9): sc-2711688	Santa Cruz Biotechnologies, sc-2711688 MW (Dhh) 42 kDa	Mouse monoclonal IgG	1:1000
Cyp11A1	Recombinant full-length protein corresponding to Human CYP11A1 aa 40–320 mapping to 10q24.32	Anti-Cypl 1A1 antibody	Abcam, Ab175408 MW (Cyp11A1) 55 kDa	Rabbit polyclonal IgG	1:1000
17ßHSD	Genetic locus: HSD17B1 (human) mapping to 17q21.2	17ß-HSD (A5)	Santa Cruz Biotechnologies, Sc-376719 MW (17ß-HSD) 35 kDa	Mouse monoclonal IgG	1:2000
ACTB	Epitope mapping at the C\ terminus of actin of human origin	Beta Actin antibody (GT5512)	GeneTex, GTX629630	Mouse monoclonal IgG	1:2000

Abbreviations: ACTB= actin; Amh (MIS) = Mullerian inhibiting substance; CPY11A1 = Cytochrome P450 side cleavage enzyme; Dhh= Desert hedgehog; HSD= hydroxysteroid dehydrogenase; MW, molecular weight