Table 1.
Prime editing by optimized pPE2 systems in transgenic plants
| Targets | pPE variants | pegRNA structures | pegRNA promotersa | Tested linesb | Precisely edited plantsc | Plants with byproductd | ||
|---|---|---|---|---|---|---|---|---|
| Ho | He | Total (%) | ||||||
| ACC-T | pPE2 | pegRNA | OsU3 | 48 | 0 | 0 | 0 (0.00%) | 0 |
| pPE2 | pegRNA-evoPreQ1 | OsU3 | 48 | 0 | 1 | 1 (2.08%) | 0 | |
| pPEmax | pegRNA-evoPreQ1 | OsU3 | 48 | 0 | 7 | 7 (14.58%) | 0 | |
| pPEmax | pegRNA-evoPreQ1 | U6 composite | 48 | 0 | 34 | 34 (70.83%) | 1 | |
| PDS-T | pPE2 | pegRNA | OsU3 | 48 | 0 | 4 | 4 (8.33%) | 0 |
| pPE2 | pegRNA-mpknot | OsU3 | 48 | 0 | 5 | 5 (10.42%) | 0 | |
| pPE2 | pegRNA-evoPreQ1 | OsU3 | 48 | 0 | 10 | 10 (20.83%) | 0 | |
| pPEmax | pegRNA-evoPreQ1 | OsU3 | 48 | 0 | 14 | 14 (29.17%) | 0 | |
| pPEmax | pegRNA-evoPreQ1 | U6 composite | 48 | 10 | 21 | 31 (64.58%) | 4 | |
| ALS-T | pPE2 | pegRNA | OsU3 | 48 | 0 | 1 | 1 (2.08%) | 0 |
| pPE2 | pegRNA-mpknot | OsU3 | 48 | 0 | 8 | 8 (16.67%) | 0 | |
| pPE2 | pegRNA-evoPreQ1 | OsU3 | 48 | 0 | 23 | 23 (47.92%) | 0 | |
| pPEmax | pegRNA-evoPreQ1 | OsU3 | 48 | 8 | 24 | 32 (66.67%) | 0 | |
| pPEmax | pegRNA-evoPreQ1 | U6 composite | 48 | 16 | 20 | 36 (75.00%) | 2 | |
| CDC48-T | pPE2 | pegRNA | OsU3 | 48 | 0 | 14 | 14 (29.17%) | 0 |
| pPE2 | pegRNA-mpknot | OsU3 | 48 | 0 | 12 | 12 (25.00%) | 0 | |
| pPE2 | pegRNA-evoPreQ1 | OsU3 | 48 | 0 | 24 | 24 (50.00%) | 0 | |
| pPEmax | pegRNA-evoPreQ1 | OsU3 | 48 | 8 | 22 | 30 (62.50%) | 0 | |
| pPEmax | pegRNA-evoPreQ1 | U6 composite | 48 | 19 | 18 | 37 (77.08%) | 0 | |
He, heterozygous mutants; Ho, homozygous mutants
aU3, OsU3 promoter; U6 composite, CaMV 35S enhancer-CmYLCV-U6 composite promoter
bFor each vector, 48 independent lines were randomly selected for genotyping
cThe number of transgenic plants harboring precise edits
dThe number of plants carrying undesired mutations in the targeted region