Table 2.

Phenotypes of osteoblast linage cells with modified STAT3 signaling pathway

Origin	Signaling pathway	Regulating factors	Effect on STAT3	Downstream target gene	Phenotypes	Reference
Mice BMSCs	JAK/STAT3	LIF	Increased STAT3 phosphorylation	OCN, BSP, ALP, COLIαI, RUNX2, OSX	Suppressed osteogenic differentiation	[43]
SHP2KOBglap osteoblast cells	RUNX2/OSTERIX, SHP2/STAT3	SHP2	Increased STAT3 phosphorylation	RANKL	Inhibited cell maturation	[52]
gp130FXXQ/FXXQ mice osteoblast	YXXQ/STAT3-dependent	IL-6	Decreased STAT3 phosphorylation	ALP	Decreased mineralization	[55]
gp130F759/F759 mice osteoblast	Y759/SHP-2-dependent negative regulatory	IL-6	Increased STAT3 phosphorylation	ALP	Increased cell differentiation	[55]
Primary bone-derived cells	JAK2/STAT3	IL-17A	Increased STAT3 phosphorylation	ALP	Increased cell differentiation and mineralized nodes	[56]
Human primary osteoblastic cells	JAK2/STAT3	PC1-CT	Increased STAT3 phosphorylation; STAT3-DNA binding	RUNX2	Increased cell differentiation	[60]
hFOB 1.19	JAK2/STAT3	EPO	Increased STAT3 phosphorylation	ALP, OCN, OPG, OPN	Stimulated osteoblast proliferation and differentiation	[70]
MC3T3-E1 cells	JAK3/STAT3	NDRG2	Increased STAT3 phosphorylation	RUNX2, OPG, OSX, ALP, OCN	Increased cell differentiation and	[62]
Osx::PKD1fl/fl calvarial preosteoblasts	STAT3	PKD1	Increased STAT3 phosphorylation	ALP, OSX, RUNX2, COL-α1	Increased cell differentiation	[64]
Mice MSCs	STAT3	HIF-1α	Increased STAT3 phosphorylation	COL1α1, RUNX2, ALP, OSX, OCN, VEGF	Increased cell differentiation and mineralization	[78]
MC3T3-E1 cells	SOCS3/STAT3	CUEDC2	Decreased STAT3 phosphorylation	ALP, RUNX2, OSX	Decreased cell differentiation	[65]
Prx1Cre; Leprfl/fl mice SSCs	JAK2/STAT3	LepR	Decreased STAT3 phosphorylation		Blocked adipocyte differentiation;	[61]
Human BMSCs	JAK1/STAT3	RPN2	Decreased STAT3 phosphorylation and nuclear location	OCN, OPN, RUNX2, BSP, ALP	Increased osteogenic differentiation and mineralized nodes	[67]
ΔTsc1 primary calvarial cells	STAT3/p63/Jagged/Notch	mTOR1	Increased STAT3 phosphorylation	RUNX2	Prevented osteoblast maturation and mineralization	[68]
MC3T3-E1 cells	JAK2/STAT3	miR-135b	Decreased JAK2 STAT3 phosphorylation	ALP	Decreased cell viability, and mineralized nodes; increased cell apoptosis	[72]
Human MSCs	STAT3/miR-7-5p/CRY2 CLOCK, BMAL1/ P300		Increased STAT3 phosphorylation	ALP, RUNX2, OCN, TYPE I COLLAGEN	Increased cell differentiation and mineralization	[74]
MC3T3-E1 cells	STAT3	miR-3074-5p,	Decreased STAT3 phosphorylation	XIAP, c-IAP2, SURVIVIN,	Promoted cell apoptosis	[73]
Human MSCs	JAK/STAT3	miR‐224	Decreased STAT3 phosphorylation	OCN, OPN, RUNX2, BSP, and ALP	Increased cell differentiation and mineralization	[77]
Human BMSCs	JAK/STAT3	OSM	Increased STAT3 phosphorylation	ALP, RUNX2	Increased cell differentiation and mineralized nodes	[109]
Vascular smooth muscle cells	STAT3/ Runx2	IL-6/IL-6R	Increased STAT3 phosphorylation	RUNX2, ALP, OPN	Increased cell osteoblast-like differentiation and mineralization	[110]