Figure 5. Lipidomic analysis of epidermal LCs.
Freshly isolated epidermal cells from IMQ– and IMQ+ mice underwent dead-cell removal and LC enrichment using microbeads. Epidermal LCs were freshly sorted from LC-enriched epidermal cell suspensions by flow cytometry. Lipids were extracted from the separated LCs and tested by LC-MS analysis. (A–F) The peak areas of differentially expressed lipid metabolites between normal and disease-associated LCs. TG, triglyceride; DG, diglyceride; PC, phosphatidylcholine; PEt, phosphatidyl ethanol. n = 13. Two-tailed Student’s t test was performed. The data are presented as mean ± SEM. *P < 0.05.