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. 2022 Jul 8;7(13):e152738. doi: 10.1172/jci.insight.152738

Figure 4. IFN-I can directly activate primary human HepSCs.

Figure 4

(A) Rested primary human HepSCs were treated with different doses of IFN-α2a. RT-qPCR was performed to measure HepSC-activation genes α-SMA, Col.1a1, and Timp1. (B and C) Rested HepSCs were treated with IFN-α2a after exposure to anti-IFNAR1 Ab or isotype control. Expression of α-SMA, Col.1a1, ISG15, OAS1, and Mx-1 was detected by RT-qPCR. (D) TGF-β is not involved in IFN-α2a–induced HepSC activation. Rested HepSCs were treated with IFN-α2a or TGF-β in the presence of isotype or anti-TGF-β Ab. Expression of Col.1a1 was detected by RT-qPCR. Data were normalized with Gapdh and represent the average of 3 independent experiments; error bars indicate the SEM. Statistical analysis was performed with 1-way ANOVA and Fisher’s LSD test. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.00005.