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HEK293T cells were transfected with (A) ZFP521-Luciferase or (B) C1qB273-Luciferase and cotransfected with a PU.1 expression vector. Cells were then treated with tBHP or cotreated with tBHP and Et-2-HOBA. SV40-Renilla-Luciferase construct was used as an expression control. Data were analyzed by 1-way ANOVA with Tukey’s post hoc test (n = 3–4, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).
