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. 2022 Jul 8;7(13):e136678. doi: 10.1172/jci.insight.136678

Figure 3. IsoLG adducts are enriched in SLE-prone mice and are efficiently scavenged by 2-HOBA.

Figure 3

Cells were isolated at the time of sacrifice from 32-week-old B6.SLE123 mice. Single-cell suspensions were prepared from freshly isolated mouse tissue via enzymatic digestion and mechanical dissociation. Live cell singlets were analyzed. (A) Representative histograms revealing isoLG adduct enrichment and efficient scavenging by 2-HOBA in DCs from spleens. Quantitation of isoLG adduct–containing cells in (B) splenic DCs and (C) splenic Ly6c+ monocytes. (D) Representative histogram from peripheral blood Ly6c+ monocytes and quantitation of (E) isoLG adduct–containing peripheral blood Ly6c+ monocytes and (F) peripheral blood total monocytes. (G) Representative histogram and quantitation of IsoLG adduct–containing (H) bone marrow and (I) splenic plasma cells. Data were analyzed using 1-way ANOVA (n = 5–9, **P < 0.01, ***P < 0.001, ****P < 0.0001).