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. 2022 Jul 11;9:913780. doi: 10.3389/fvets.2022.913780

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Copyright © 2022 Huang, Liu, He, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding sites of RPA primers and crRNA oligonucleotides targeting to ORF-1 gene within the FCV genome map. crRNA1, crRNA2, and RPA primers were designed in positive and negative strands of the FCV ORF1 gene (from 2418-2550 nt), respectively, according to the conserved sequence of epidemic FCV strain SMU-F4-2020 from Southwest China.