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Validation of FCV-Cas13a assays. (A) Detection results of FCV-Cas13a-LFD and FCV-Cas13a-FLOUR. Visual readouts of seven RSs with different components was recorded by endpoint imaging of lateral flow dipstick or fluorescence detector. (B) Fluorescence plottings of seven RSs by FCV-Cas13a-FLUOR. Both RS6 and RS7 were valid, but RS7, by contrast, was more robust. Each experiment was repeated three times.
