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. 2022 Jul 11;9:913780. doi: 10.3389/fvets.2022.913780

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Copyright © 2022 Huang, Liu, He, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specificity testing of FCV-Cas13a assays. (A) Detection results of FCV-Cas13a-LFD and FCV-Cas13a-FLUOR. Both methods only detected FCV RNA but no other pathogens. (B) Electrophoresis of RPA amplicons of all tested pathogens. Lane M, standard molecular weight; lane NC, negative control (RNase-Free distilled water). (C) Detection results by FCV-Cas13a-FLUOR were shown in fluorescence plottings. Each experiment was repeated three times.