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. 2022 Jul 11;9:913780. doi: 10.3389/fvets.2022.913780

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Copyright © 2022 Huang, Liu, He, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sensitivity testing of FCV-Cas13a assays. (A) Detection results of FCV-Cas13a-LFD and FCV-Cas13a-FLUOR. Gradiently diluted FCV plasmid DNAs were detected using FCV-Cas13a assays. The minimum detection limit was 5.5 copies/μl. (B) RT-qPCR amplification curves. No amplification curve was recorded when the plasmid DNA concentration was lower than 5.5 ×10² copies/μl. (C) Detection results by FCV-Cas13a-FLUOR were shown in fluorescence profiles. Each experiment was repeated three times. NC, Negative control (RNase-Free distilled water).