Figure 5.
Effect of GHRH on the promotion of tumorigenesis. (A) RWPE‐1 cells were incubated in the absence or the presence of 0.1 µM GHRH for 24 h. The cell suspension was mixed with Matrigel synthetic basement membrane and then injected under the skin in the right flank (1 × 107 cells/mouse). Seven animals were used per group. Experiment lasted for 49 days. Data in each bar are the means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 versus control. (B) Image from a representative animal of each group. Tumor mass is marked with a black arrow. GHRH, growth hormone‐releasing hormone; GHRH, growth hormone‐releasing hormone [Color figure can be viewed at wileyonlinelibrary.com]